17-10456
ChIPAb+ REST - ChIP Validated Antibody and Primer Set
from rabbit
Synonym(s):
Chip Antibody and primer set, Neural-restrictive silencer factor ChIP, Neural-restrictive silencer factor, RE1-silencing transcription factor, X2 box repressor, neuron restrictive silencer factor, repressor binding to the X2 box, RE1-silencing transcription factor ChIP, REST ChIP, X2 box repressor ChIP
About This Item
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General description
The ChIPAb+ REST set includes the REST antibody, a Normal Rabbit IgG, and control primers which amplify a 89 bp region of the human Snap25 promoter. The REST and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of REST associated chromatin.
Specificity
Immunogen
Application
Sonicated chromatin prepared from HeLa cells (4e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either Normal Rabbit IgG (Part No. PP64B), or 4 µg Anti-REST (Part No.CS207378) and the Magna ChIP HiSens (Cat. # 17-10460). Successful immunoprecipitation of REST associated DNA fragments was verified by qPCR using ChIP Primers, human Snap25 (Part No. CS207379) as a positive locus, and CDC25 as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Western Blotting Analysis:
Human HEK293 cell lysate (30 ug) was resolved by electrophoresis, transferred to PVDF membrane and probed with the anti-REST (1:1000 dilution). Proteins were visualized using a secondary antibody conjugated to HRP and a chemiluminescence detection system. Data courtesy of Glen Corson laboratory of Gail Mandel, Oregon Health and Sciences University, Portland, OR.
REST has a predicted molecular weight of 125 kDa. Observed size was ~200 kDa.
Epigenetics & Nuclear Function
Chromatin Biology
Histones
Packaging
Quality
Sonicated chromatin prepared from HeLa cells (4e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either Normal Rabbit IgG (Part No. PP64B), or 4 µg Anti-REST (Part No.CS207378) and the Magna ChIP® HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of REST associated DNA fragments was verified by qPCR using ChIP Primers, human Snap25.
Target description
Physical form
Normal Rabbit IgG. One vial containing 125 µg Rabbit IgG in 125 µL storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, human Snap25. One vial containing 75 μL of 5 μM of each primer specific for human Snap25 promoter. Store at -20°C.
FOR: AGA CTC CTT TGC AGA CAA TTT CCT
REV: CAA CAC AGA AGA TTT CCA CAA GTA GAC
Storage and Stability
Analysis Note
Includes normal rabbit IgG and primers specific for human Snap25.
Legal Information
Disclaimer
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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