178488

Apoptosis Inhibitor

The Apoptosis Inhibitor, also referenced under CAS 54135-60-3, controls the biological activity of Apoptosis. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Apoptosis Inhibitor, M50054, 2,2ʹ-Methylenebis(1,3-cyclohexanedione)
• Empirical Formula (Hill Notation): C₁₃H₁₆O₄
• CAS Number: 54135-60-3
• Molecular Weight: 236.26
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: off-white
• solubility: DMSO: 100 mg/mL; ethanol: 20 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C13H16O4/c14-10-3-1-4-11(15)8(10)7-9-12(16)5-2-6-13(9)17/h8-9H,1-7H2
• InChI key: ILUMEPMGPCKGHH-UHFFFAOYSA-N

Description

General description

A cell-permeable inhibitor of apoptosis induction (IC₅₀ = 67 µg/ml in FasL-stimulated WC8 cells, 130 µg/ml in etoposide-stimulated U937 cells). Effects attributable to the inhibition of caspase-3 activation but does not directly inhibit caspase-3 even at 1 mg/ml.

A cell-permeable, bis(cyclohexane-1,3-diketone) that is shown to inhibit apoptosis induction in FasL-stimulated WC8 cells (IC₅₀ = 67 µg/ml) and etoposide-stimulated U937 cells (IC₅₀ = 130 µg/ml). The anti-apoptotic effects have been shown to be attributable to inhibition of caspase-3 activation (IC₅₀ = 334 nM), however it does not exhibit any direct inhibition of caspase-3 enzymatic activity. Shown to inhibit the elevation of the liver enzymes, alanine aminotransferase and aspartate aminotransferase, in a murine model of anti-Fas-induced hepatitis. Topical application has been shown to reduce chemotherapy-induced alopecia (hair loss) in newborn rats.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
apoptosis induction

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 67 µg/ml in FasL-stimulated WC8 cells, 130 µg/ml in etoposide-stimulated U937 cells

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Tsuda, T., et al. 2001. Eur. J. Pharmacol.433, 37.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable