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196810

Sigma-Aldrich

Bax-Inhibiting Peptide, V5

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection.

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Synonym(s):
Bax-Inhibiting Peptide, V5, BIP-V5, H-VPMLK-OH
Empirical Formula (Hill Notation):
C27H50N6O6S
Molecular Weight:
586.79

Quality Level

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

white

solubility

water: 1 mg/mL

shipped in

wet ice

storage temp.

−20°C

General description

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection. Functions as effectively as the Caspase Inhibitor VI (Z-VAD-FMK; Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also effectively blocks caspase-independent necrotic cell death. Shown to be Ku70 competitive, interact with Bax, prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days). Negative control peptide is also available (Cat. No. 196811).
A cell-permeable pentapeptide derived from the Ku70-Bax inhibiting domain that has been shown to offer protection against apoptosis in cells of human, mouse, rant, and porcine origin. Reported to function as effectively as the caspase inhibitor, Z-VAD-FMK (Caspase Inhibitor VI, Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also reported to effectively block caspase-independent necrotic cell death. Shown to interact with Bax in a Ku70-competitive manner and prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days).

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Bax mediated apoptosis
Product does not compete with ATP.
Reversible: no
Target IC50: 50-200 µM against Bax mediated apoptosis

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Val-Pro-Met-Leu-Lys-OH

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.

Other Notes

Yoshida, T., et al. 2004. Biochem. Biophys. Res. Commun.321, 961.
Sawada, M., et al. 2003. Nature Cell Biol.5, 352.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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