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219475

Sigma-Aldrich

CENTA β-Lactamase Substrate

CENTA β-Lactamase Substrate, CAS 9073-60-3, is a chromogenic β-lactamase substrate for both Gram-negative and Gram-positive bacteria.

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Synonym(s):
CENTA β-Lactamase Substrate
Empirical Formula (Hill Notation):
C21H17N3O8S3
CAS Number:
Molecular Weight:
535.57
MDL number:

Quality Level

assay

≥98% (TLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

yellow

solubility

25 mM phosphate buffer, pH 7.0: 2.5 mg/mL
methanol: 50 mg/mL

shipped in

ambient

storage temp.

−20°C

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CENTA β-Lactamase Substrate CENTA β-Lactamase Substrate, CAS 9073-60-3, is a chromogenic β-lactamase substrate for both Gram-negative and Gram-positive bacteria.

219475

CENTA β-Lactamase Substrate

Sigma-Aldrich

L6170

β-Lactamase

Penicillinase from Bacillus cereus lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)

P0389

Penicillinase from Bacillus cereus

form

powder

form

lyophilized powder

form

powder

form

lyophilized powder

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

25 mM phosphate buffer, pH 7.0: 2.5 mg/mL, methanol: 50 mg/mL

solubility

-

solubility

-

solubility

-

General description

Chromogenic β-lactamase substrate for both gram-negative and gram-positive bacteria that is comparable to other chromogens (PADAC Substrate and nitrocefin). Hydrolysis of the beta-lactam ring causes a color change from light yellow (λmax: 340 nm) to chrome yellow (λmax: 405 nm). Relatively unaffected by commonly used microbiological media and human serum. Stable in H2O and in the presence of cysteine, mercaptoethanol and dimercaptopropanol.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Chromogenic β-lactamase substrate for both gram-negative and gram-positive bacteria that is comparable to other chromogens (PADAC and nitrocefin). Hydrolysis of the beta-lactam ring causes a color change from light yellow (λmax: 340 nm) to chrome yellow (λmax: 405 nm). Relatively unaffected by commonly used microbiological media and human serum. Stable in H2O and in the presence of cysteine, mercaptoethanol, and dimercaptopropanol.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Jones, R.N., et al. 1982. J. Clin. Microbiol.15, 954.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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