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Ethidium Bromide Adsorber

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.

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Synonym(s):
Ethidium Bromide Adsorber
CAS Number:

Quality Level

form

suspension

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

gray-brown

shipped in

ambient

storage temp.

15-25°C

InChI

1S/C10H12.C10H10.C8H8/c2*1-3-9-7-5-6-8-10(9)4-2;1-2-8-6-4-3-5-7-8/h3,5-8H,1,4H2,2H3;3-8H,1-2H2;2-7H,1H2

InChI key

NWUYHJFMYQTDRP-UHFFFAOYSA-N

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E1385E1510E7637
Ethidium bromide solution BioReagent, for molecular biology, 500 μg/mL in H2O

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E1385

Ethidium bromide solution

Essential+ Grade
Ethidium bromide solution BioReagent, for molecular biology, 10 mg/mL in H2O

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E1510

Ethidium bromide solution

Essential+ Grade
Ethidium bromide BioReagent, for molecular biology, powder

Sigma-Aldrich

E7637

Ethidium bromide

Essential+ Grade
manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

color

gray-brown

color

-

color

-

color

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

storage temp.

15-25°C

storage temp.

-

storage temp.

-

storage temp.

-

General description

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5-10 mg/l. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Irritant (B)

Reconstitution

When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.

Other Notes

MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103.
Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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