343222

Biotin, Fluorescein Conjugate

• UNSPSC Code: 41116133
• NACRES: NA.41

Properties

• form: lyophilized solid
• Quality Level: 100
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: orange
• solubility: DMF: 5 mg/mL; DMSO: 5 mg/mL; methanol: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

It can be used for the detection of avidin and streptavidin.

Application

EIA (1:1,000)

Tissue Staining (1:50 - 1:100)

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Other Notes

Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203

Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable