344822

α1,2-Fucosidase, Xanthomonas sp.

Catalyzes the hydrolysis of all non-reducing terminal fucose that are α1,2-linked to the galactose of terminal Gal-GlcNAc disaccharide structures.

• Synonym(s): α1,2-Fucosidase, Xanthomonas sp., α-L-Fucoside Fucohydrolase
• CAS Number: 9037-65-4
• Enzyme Commission number: 3.2.1.51 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: bacterial (Xanthomonas spp.)
• Quality Level: 100
• form: liquid
• specific activity: ≥0.4 units/mg protein; ≥100 mU/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: β-galactosidase, β-hexosaminidase, α- and β-mannosidase, neuraminidases, proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Native α1,2-fucosidase from Xanthomon as species. Catalyzes the hydrolysis of all non-reducing terminal fucose that are α1,2-linked to the galactose of terminal Gal-GlcNAc disaccharide structures. The enzyme will not cleave fucose linked to the GlcNAc residue.

Note: 1 mU = 1 milliunit.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol fucose from 2ʹ-fucosyllactose per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

Noronkoski, T., and Mononen, I. 1997. Glycobiology 7, 217.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable