420290

IPTG, Hemidioxane Adduct

A free-flowing, hygroscopic carbohydrate that induces expression of the lac operon of E. coli by allosterically interacting with the repressor molecule.

• Synonym(s): IPTG, Hemidioxane Adduct, Isopropyl-β-D-thiogalactopyranoside
• Empirical Formula (Hill Notation): C₉H₁₈O₅S · 0.5C₄H₈O₂
• CAS Number: 367-93-1
• Molecular Weight: 282.35
• MDL number: MFCD00063273
• UNSPSC Code: 12352201
• NACRES: NA.25

Properties

• Quality Level: 100
• assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• impurities: 15.0% dioxane
• color: white to off-white
• solubility: water: 1%; ethanol: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Description

General description

A free flowing carbohydrate that induces expression of the lac operon of E. coli by allosterically interacting with the repressor molecule. Inducer of β-D-galactosidase.

A free-flowing, hygroscopic carbohydrate that induces expression of the lac operon of E. coli by allosterically interacting with the repressor molecule. Inducer of β-D-galactosidase. This preparation contains 15% dioxane.

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
White, R.J., et al. 1996. J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995. Neuron 15, 961.
Reers, M., et al. 1995. Methods Enzymol. 260, 406.
Ravanello, M.P., and Kruby, D.E. 1994. J. Virol.68, 6401.
Schmitz, M.L., and Baeurerle, P.A. 1994. Biotechniques17, 714.
Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.
Lama, J., et al. 1992. Gene117, 185.
Zhang, Y., and Moss, B. 1991. Proc. Natl. Acad. Sci. USA88, 1511.
Rodriquez, J.F., and Smith, G.L. 1990. Nucleic Acids Res.18, 5347.
Date, T., et al. 1988. Biochem. Biophys. Res. Commun.151, 598.
Lee, S.G., et al. 1988. Biochemistry27, 2983.
Cho, S., et al. 1985. Biochem. Biophys. Res. Commun.128, 1268.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable