5.30030

Glutaminase Inhibitor II, BPTES

• Synonym(s): Glutaminase Inhibitor II, BPTES, N,Nʹ-(5,5ʹ-(2,2ʹ-Thio bis(ethane-2,1-diyl)) bis(1,3,4-thiadiazole-5,2-diyl)) bis(2-phenylacetamide), Kidney-Type Glutaminase Inhibitor II, GAC Inhibitor II, GLS1 Inhibitor II, KGA Inhibitor II
• Empirical Formula (Hill Notation): C₂₄H₂₄N₆O₂S₃
• CAS Number: 314045-39-1
• Molecular Weight: 524.68
• MDL number: MFCD01079848
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• assay: ≥94% (HPLC)
• Quality Level: 100
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 50 mg/mL
• storage temp.: −20°C
• InChI: 1S/C24H24N6O2S3/c31-19(15-17-7-3-1-4-8-17)25-23-29-27-21(34-23)11-13-33-14-12-22-28-30-24(35-22)26-20(32)16-18-9-5-2-6-10-18/h1-10H,11-16H2,(H,25,29,31)(H,26,30,32)
• InChI key: MDJIPXYRSZHCFS-UHFFFAOYSA-N

Description

General description

Glutaminase Inhibitor II,Bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide (BPTES)is a cell-permeable bis-thiadiazole compound that selectivelyinhibits glutaminase-1(GLS1). It does not inhibit the liver-type GLS-2 mitochondrialglutaminase activity. It exerts its effect by inducing an inactive GLS1tetrameric conformation via a 2:1 inhibitor-to-tetramer stoichiometric binding.BPTES blocks the growth under aerobic/ normoxic conditions, and promotes celldeath under hypoxic conditions in P493 B-cell lymphoma cultures invitro, and effectively suppresses P493 tumor expansion in mice invivo.BPTES has been observedto be active in several cancer cells as it prevents cancer cellproliferation.

Application

Glutaminase Inhibitor II, BPTES has been used asa glutaminase inhibitor to study its effects on the release of extracellularvesicles (EVs) in human immunodeficiency virus (HIV-1) infected macrophages.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
kidney-type glutaminase

Reversible: yes

Target IC₅₀: 140 and 210 nM in HEK293 cells expressing wt-KGA and F318Y-cKGA, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Shukla, K., et al. 2012. J. Med. Chem.55, 10551.

Thangavelu, K., et al. 2012. Proc. Natl. Acad. Sci. USA109, 7705.
Le, A., et al. 2012. Cell Metab.15, 110.
DeLaBarre, B., et al. 2011. Biochemistry 50, 10764.
Seltzer, M.J., et al. 2010. Cancer Res.70, 8981.
Robinson, M.M., et al. 2007. Biochem. J.406, 407.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315 - H319 - H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable