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usage
sufficient for 10 dissociation reaction(s) (1 g each)
Quality Level
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
input
sample type: eukaryotic fresh tissue sample(s)
shipped in
ambient
storage temp.
2-8°C
General description
The ProteoExtract Tissue Dissociation Buffer Kit is a tool for isolation of viable cells from fresh tissue. The isolated cells can then be used for total, subcellular, membrane, or other form of proteome extractions. This kit serves as an accessory product for the following kits: ProteoExtract Subcellular Proteome Extraction Kit (Cat. No.: 539790), ProteoExtract Subcellular Proteome Extraction Kit, Mini (Cat. No.: 539791), ProteoExtract Native Membrane Protein Extraction Kit (Cat. No.: 444810).
Components
10X Tissue Dissociation Buffer, Protease Inhibitor Cocktail, Tissue Dissociation Buffer Additive and a user protocol.
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Principle
The Calbiochem ProteoExtract Tissue Dissociation Buffer Kit is a tool for isolation of viable cells from fresh tissue for the purpose of protein extraction. It is an accessory product for the following kits:
• ProteoExtract Subcellular Proteome Extraction Kit (S-PEK, Cat. No. 539790)
• ProteoExtract Subcellular Proteome Extraction Kit, Mini (S-PEK Mini, Cat. No. 539791)
• ProteoExtract Native Membrane Protein Extraction Kit (M-PEK, Cat. No. 444810).
With the exception of the collagenase enzyme the set contains all reagents required for efficient dissociation of tissue samples. The goal of the cell isolation procedure is to maximize the yield of viable dissociated cells, so the end-user must select the collagenase appropriate for each tissue with respect to the type of tissue, the species of origin, and the age of the animal. A list of enzymes is provided below in Materials Required but Not Provided.
• ProteoExtract Subcellular Proteome Extraction Kit (S-PEK, Cat. No. 539790)
• ProteoExtract Subcellular Proteome Extraction Kit, Mini (S-PEK Mini, Cat. No. 539791)
• ProteoExtract Native Membrane Protein Extraction Kit (M-PEK, Cat. No. 444810).
With the exception of the collagenase enzyme the set contains all reagents required for efficient dissociation of tissue samples. The goal of the cell isolation procedure is to maximize the yield of viable dissociated cells, so the end-user must select the collagenase appropriate for each tissue with respect to the type of tissue, the species of origin, and the age of the animal. A list of enzymes is provided below in Materials Required but Not Provided.
Preparation Note
• 1X Tissue Dissociation Buffer: Prepare 50 ml 1X Tissue Dissociation Buffer by adding 5 ml 10X Tissue Dissociation Buffer to 45 ml high quality water. Add 8.5 µl Tissue Dissociation Buffer Additive and mix. • Tissue Dissociation Solution: Dissolve an appropriate amount of collagenase in 10 ml 1X Tissue Dissociation Buffer just prior to use.• 1X Tissue Dissociation Buffer with Protease Inhibitors: Add 100 µl Protease Inhibitor Cocktail to the remaining 40 ml 1X Tissue Dissociation Buffer and mix.
Storage and Stability
Upon arrival store the entire contents of the kit at 4°C. Following reconstitution, all components are stable for a minimum of 2 days at 4°C or for up to 2 months at -20°C. Avoid freeze/thaw cycles of reconstituted solutions.
Analysis Note
Table 2: Calculating Number of Cells
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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