648317

Tris, Hydrochloride, Molecular Biology Grade

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

• Synonym(s): Trizma^® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein/REAXYS Number: 3675235
• MDL number: MFCD00012590
• UNSPSC Code: 12161705
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 100
• description: RTECS - TY2900000
• assay: ≥99% (titration)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1

Description

General description

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used biological buffer (absorbance: ≤0.05 at 260 nm and 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Warning

Toxicity: Standard Handling (A)

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable