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676604

Sigma-Aldrich

PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

lyophilized, clone 5C6, Calbiochem®

Synonym(s):

Anti-Vasodilator-stimulated Phosphoprotein, Activated

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100 μG
$691.00

$691.00


Estimated to ship onMarch 23, 2025


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100 μG
$691.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

$691.00


Estimated to ship onMarch 23, 2025


Request a Bulk Order

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

5C6, monoclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

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This Item
SAB4200521AP1064676602
clone

5C6, monoclonal

clone

polyclonal

clone

polyclonal

clone

16C2, monoclonal

Gene Information

human ... VASP(7408)

Gene Information

human ... VASP(7408)
mouse ... Vasp(22323)
rat ... Vasp(361517)

Gene Information

-

Gene Information

-

species reactivity

human, mouse

species reactivity

mouse, human, rat

species reactivity

human

species reactivity

mouse, human

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

General description

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
This PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP

Application

ELISA (0.05 µg/ml)

Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from PBS, PEG, sucrose.

Reconstitution

Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Vanadate-treated A431 cells

Other Notes

Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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    Min Li et al.
    The Journal of biological chemistry, 290(50), 30030-30041 (2015-10-27)
    Diabetes is strongly associated with cognitive decline, but the molecular reasons are unknown. We found that fasting and peripheral insulin promote phosphorylation and dephosphorylation, respectively, of specific residues on brain proteins including cytoskeletal regulators such as slit-robo GTPase-activating protein 3

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