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Sigma-Aldrich

Caspase-1 Substrate VI, Fluorogenic

Fluorogenic caspase-1 substrate.

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Synonym(s):
Caspase-1 Substrate VI, Fluorogenic, Z-YVAD-AFC
Empirical Formula (Hill Notation):
C33H36F3N5O10
CAS Number:
Molecular Weight:
719.66

Quality Level

assay

98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

white to off-white

solubility

DMSO: 5 mg/mL

fluorescence

λex ~400 nm
λem ~505 nm

shipped in

ambient

storage temp.

−20°C

InChI

1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1

InChI key

GWKJISJKNKIJNP-DEQDFGQZSA-N

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Sigma-Aldrich

218739

Caspase-1 Substrate X, Fluorogenic

assay

98% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, protect from light, desiccated

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light

General description

Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.
Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Fluorogenic caspase-1 substrate
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Tyr-Val-Ala-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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