69453-M

HMS174(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

• Synonym(s): DE3 Competent Cells
• UNSPSC Code: 41106202
• NACRES: NA.81

Properties

• product name: HMS174(DE3) Competent Cells - Novagen, HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
• biological source: Escherichia coli
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: transformation efficiency: >5×10⁶ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Genotype: F^-recA1 hsdR(r_K12^- m_K12⁺) (DE3) (Rif ^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

HMS174 strains provide the recA mutation in aK-12 background. Like BLR, these strains maystabilize certain target genes whose productsmay cause the loss of the DE3 prophage.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlHMS174(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2