70018-M

T7Select^® Biopanning Kit

Matched reagents for selecting binders from T7Select libraries

• UNSPSC Code: 41105500
• NACRES: NA.51

Properties

• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles

Description

General description

Enrichment of T7 phage displaying target peptides or proteins can easily be accomplished with the T7Select^® Biopanning Kit. The kit contains reagents to perform biopanning with target ligands using 96-well microtiter plates or similar solid phases. Biopanning procedures can be tested and optimized using the positive and negative controls provided in the kit. The supplied Positive Control Lysate contains a T7Select415-1 recombinant that displays the S•Tag peptide. When combined with the Negative Control Lysate at known ratios, the degree of enrichment of high affinity binding phage can be monitored by a simple plaque lift assay with the provided detection reagents.
Biopanning selection
Biopanning requires both binding and elution conditions that maintain the infectivity of the phage. The stability of the T7 phage particle enables the use of a variety of elution conditions during biopanning. Phage maintain infectivity following treatment with 1% SDS, 5 M NaCl, 4 M urea, 2 M guanidine-HCl, 10 mM EDTA, reducing conditions (up to 100 mM DTT), and a pH range of 4 to 10. The wide range of conditions available for T7Select biopanning expands the range of targets that can be used successfully.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Components

• 25 g Blocking Reagent
• 2 x 50 ml 10X TBST Wash Buffer
• 10 ml T7 Elution Buffer
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BLT5403 Glycerol Stock
• 0.2 ml BLT5615 Glycerol Stock
• 10¹⁰ pfu T7Select Positive Control Lysate
• 10¹⁰ pfu T7Select Negative Control Lysate
• 50 μl S-protein HRP Conjugate
• 2 ml T7Select S-protein
• 50 ml SuperSignal Substrate
• pkg/25 g LOCATOR Luminescent Labels
• pkg/25 Development Folders

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3