70625

Tuner (DE3)pLacI Competent Cells - Novagen^®

Escherichia coli, rod shaped

• UNSPSC Code: 41106202
• NACRES: NA.81

Properties

• product name: Tuner (DE3)pLacI Competent Cells - Novagen^®, Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. Contains the pLacI plasmid producing extra Lac repressor.
• biological source: Escherichia coli
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: transformation efficiency: >2×10⁶ cfu/μg
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Genotype: F^–ompT hsdS_B (r_B^– m_B^–) gal dcm lacY1(DE3) pLacI (Cam^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

Note that these strains are not recommended for use with pET, pACYCDuet, pCDF, pRSF, pCOLADuet or pETcoco vectors.

.Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins. In Tuner(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. Contains the pLacI plasmid producing extra Lac repressor.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlTuner(DE3)pLacI Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2