AB10546

Anti-Bcl-2-associated transcription factor 1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): BCL2-associated transcription factor 1, bcl-2-associated transcription factor 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): bovine (based on 100% sequence homology), canine (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_055554
• UniProt accession no.: Q9NYF8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BCLAF1(9774)

Description

General description

Bcl-2-associated transcription factor 1 (Btf) is a ubiquitously expressed death-promoting transcriptional repressor. It has been shown to interact with anti-apoptotic proteins related to Bcl-2, DNA, adenovirus E1B 19K, and EMD. Btf has also been shown to be involved in E1A transformation of p53 or E1B 19K, an interaction that implicates Btf as being involved in transcriptional regulation and in promoting apoptosis. Expression of Btf is absent in some cancers, which may suggest a potential role for the protein in tumor suppression.

Specificity

Other homologies: Rat (81% sequence homology).

Immunogen

KLH-conjugated linear peptide corresponding to human Bcl-2-associated transcription factor 1.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

This Anti-Bcl-2-associated transcription factor 1 Antibody is validated for use in WB, IP, IH(P) for the detection of Bcl-2-associated transcription factor 1.

Western Blot (Snap i.d.) Analysis: 0.5 µg/mL from a previous lot detected Bcl-2-associated transcription factor 1 in 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Bcl-2-associated transcription factor 1 from HeLa cell lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Bcl-2-associated transcription factor 1 in neuronal and glial cells in normal human brain tissue.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Bcl-2-associated transcription factor 1 in 10 µg of HeLa cell lysate.

Target description

~120 kDa observed.
This protein has 4 isoforms: Isoform 1 (106 kDa calculated), Isoform 2 (106 kda calculated), Isoform 3 (100 kDa calculated), Isoform 4 (86 kDa calculated).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable