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AB1513

Sigma-Aldrich

Anti-Brain Derived Neurotrophic Factor Antibody

serum, Chemicon®

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Synonym(s):
BDNF
eCl@ss:
32160702
NACRES:
NA.41

biological source

sheep

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BDNF(627)

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This Item
AB1779AB1779SPAB1513P
biological source

sheep

biological source

rabbit

biological source

rabbit

biological source

sheep

antibody form

serum

antibody form

serum

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

Gene Information

human ... BDNF(627)

Gene Information

human ... BDNF(627)

Gene Information

human ... BDNF(627)

Gene Information

human ... BDNF(627)

technique(s)

ELISA: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

species reactivity

human, rat

species reactivity

human, rat

species reactivity

rat, human

species reactivity

human, rat, chicken

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Specificity

Brain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4.

The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.

Immunogen

Recombinant Human Brain Derived Neurotrophic Factor (BDNF).

Application

Anti-Brain Derived Neurotrophic Factor Antibody is an antibody against Brain Derived Neurotrophic Factor for use in ELISA, WB, IH.
Immunohistochemistry: 1:200-1:2,000 (see suggested protocol).

Immunoblotting: 1:200-1:2,000 we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochemistry 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH 7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C.

ELISA: 1:200-1:2,000

Inhibition of biological activity in vitro: 1:10-1:50

Use neat for in vivo animal studies.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins

Neuroinflammation & Pain

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any insoluble material.

Storage and Stability

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids


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