All Photos(3)
Synonym(s):
alpha-synuclein, Alpha-synuclein
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SNCA(6622)
General description
alpha-Synuclein is a small (140 amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer′s Disease (AD). α-Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Recent studies have shown that abnormal aggregation and accumulation of synaptic proteins, including α-Synuclein might be associated with plaque formation in AD and seems to be a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA).
Specificity
Alpha-synuclein. The immunizing peptide was chosen to reduce cross-reactivity of AB5038 with β-synuclein to a minimum. Immunohistochemical and Western blot analysis of human brain indicates a high level of specificity.
Has not been tested on other species.
Immunogen
Epitope: a.a. 111-131
Human alpha-synuclein residue 111-131 coupled to KLH.
Application
Anti-Synuclein Antibody, α detects level of Synuclein & has been published & validated for use in IC, IH, IH(P) & WB.
Immunohistochemistry:
1:1,000-1:2,000 dilutoin of a previous lot was used on paraffin sections; 1:4,000-1:12,000 dilution of a previous lot was used on frozen sections.
Western blot:
1:1,000-1:2,000. Shows a 14-19kDa band.
Immunocytochemistry:
Previous lot used IC.
Optimal working dilutions must be determined by end user.
1:1,000-1:2,000 dilutoin of a previous lot was used on paraffin sections; 1:4,000-1:12,000 dilution of a previous lot was used on frozen sections.
Western blot:
1:1,000-1:2,000. Shows a 14-19kDa band.
Immunocytochemistry:
Previous lot used IC.
Optimal working dilutions must be determined by end user.
Quality
Evaluated by Western Blot on Human Brain lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected Synuclein on 10 µg of human brain lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected Synuclein on 10 µg of human brain lysates.
Target description
~14-19 kDa
Linkage
Replaces: 04-1053
Physical form
Rabbit polyclonal serum lyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any residue.
Analysis Note
Control
Human brain sections.
Human brain sections.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
recommended
Product No.
Description
Pricing
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
TDP-43 pathology in the basal forebrain and hypothalamus of patients with amyotrophic lateral sclerosis.
Cykowski, MD; Takei, H; Schulz, PE; Appel, SH; Powell, SZ
Acta Neuropathologica Communications null
Dora Games et al.
The American journal of pathology, 182(3), 940-953 (2013-01-15)
Progressive accumulation of α-synuclein (α-syn) in limbic and striatonigral systems is associated with the neurodegenerative processes in dementia with Lewy bodies (DLB) and Parkinson's disease (PD). The murine Thy-1 (mThy1)-α-syn transgenic (tg) model recapitulates aspects of degenerative processes associated with
Mitochondrial quality, dynamics and functional capacity in Parkinson's disease cybrid cell lines selected for Lewy body expression.
Cronin-Furman, EN; Borland, MK; Bergquist, KE; Bennett, JP; Trimmer, PA
Mol. Neurodegener. null
Chiara Maria Giulia De Luca et al.
Translational neurodegeneration, 8, 24-24 (2019-08-14)
Parkinson's disease (PD) is a neurodegenerative disorder whose diagnosis is often challenging because symptoms may overlap with neurodegenerative parkinsonisms. PD is characterized by intraneuronal accumulation of abnormal α-synuclein in brainstem while neurodegenerative parkinsonisms might be associated with accumulation of either
Serine 129 phosphorylation of alpha-synuclein induces unfolded protein response-mediated cell death.
Sugeno, N; Takeda, A; Hasegawa, T; Kobayashi, M; Kikuchi, A; Mori, F; Wakabayashi, K; Itoyama, Y
The Journal of Biological Chemistry null
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