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AB5543

Sigma-Aldrich

Anti-MAP2 Antibody

Chemicon®, from chicken

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Synonym(s):
Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Map2(25595)

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This Item
M4403M2320M9942
vibrant-m

AB5543

Anti-MAP2 Antibody

biological source

chicken

biological source

mouse

biological source

mouse

biological source

mouse

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

NCBI accession no.

NM_001039538.1, NM_031845.2, NM_002374.3, NM_031847.2

NCBI accession no.

-

NCBI accession no.

-

NCBI accession no.

-

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Specificity

Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals.

Immunogen

Purified bovine brain MAP2

Application

Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism
Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.

Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.

Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Target description

Recognizes a doublet of ~280 kDa and a doublet of ~70kDa

Physical form

Ammonium Sulfate Precipitation
Format: Purified
Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive = cerebral cortex. Negative = liver or kidney.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Stimulation of Glia Reveals Modulation of Mammalian Spinal Motor Networks by Adenosine.
Acton, D; Miles, GB
Testing null
Riikka H Hämäläinen et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(38), E3622-E3630 (2013-09-05)
Mitochondrial DNA (mtDNA) mutations manifest with vast clinical heterogeneity. The molecular basis of this variability is mostly unknown because the lack of model systems has hampered mechanistic studies. We generated induced pluripotent stem cells from patients carrying the most common
Yumiko V Taguchi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(40), 9617-9631 (2017-08-30)
Glucocerebrosidase 1 (GBA) mutations responsible for Gaucher disease (GD) are the most common genetic risk factor for Parkinson's disease (PD). Although the genetic link between GD and PD is well established, the underlying molecular mechanism(s) are not well understood. We
Leanne J M Schmitz et al.
eLife, 6 (2017-12-05)
Glutamatergic synapses rely on AMPA receptors (AMPARs) for fast synaptic transmission and plasticity. AMPAR auxiliary proteins regulate receptor trafficking, and modulate receptor mobility and its biophysical properties. The AMPAR auxiliary protein Shisa7 (CKAMP59) has been shown to interact with AMPARs
M Catarina Silva et al.
eLife, 8 (2019-03-26)
Tauopathies are neurodegenerative diseases characterized by aberrant forms of tau protein accumulation leading to neuronal death in focal brain areas. Positron emission tomography (PET) tracers that bind to pathological tau are used in diagnosis, but there are no current therapies

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