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AB9730

Sigma-Aldrich

Anti-14-3-3 β Antibody

serum, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rabbit, bovine, human, rat, mouse, sheep

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... YWHAB(7529)

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This Item
SAB4503099AB9746AB9738
vibrant-m

AB9730

Anti-14-3-3 β Antibody

vibrant-m

AB9746

Anti-14-3-3 ζ Antibody

vibrant-m

AB9738

Anti-14-3-3 γ Antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

serum

antibody form

affinity isolated antibody

antibody form

serum

antibody form

serum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rabbit, bovine, human, rat, mouse, sheep

species reactivity

mouse, human, rat

species reactivity

mouse, bovine, rabbit, human, rat, chicken, sheep

species reactivity

human, rat, sheep, chicken, mouse, bovine

technique(s)

ELISA: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

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General description

Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s.

The 14-3-3 beta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s Disease.

Specificity

14-3-3 beta. By Western blot the antibody recognizes the ~30 kDa protein.

Immunogen

Synthetic peptide from the N-terminal area of sheep 14-3-3 beta.

Application

Detect 14-3-3 β using this Anti-14-3-3 β Antibody validated for use in ELISA, WB, IH.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: 1:3,000 using ECL
Immunohistochemistry: 1:300-1:600 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
ELISA

Optimal working dilutions must be determined by end user.

Target description

30 kDa

Linkage

Replaces: 04-1089

Physical form

Liquid.
Serum

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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