ABE1361
Anti-NuMA1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Nuclear mitotic apparatus protein 1, NuMA protein, SP-H antigen
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About This Item
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NUMA1(4926)
General description
NuMA1 or NuMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NuMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NuMA1 resides in the nuclear matrix during interphase. NuMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NuMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis.
Specificity
Recognizes NuMA1
Immunogen
Three different peptides corresponding to multiple locations of NuMA1 isoform 1. Peptide, region between amino acids 1-240. Peptide 2, amino acids 1300-1500. Peptide 3, amino acids 1860-2115. Note that these peptides are also found in isoform 2.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot was reported to detect NuMA1 in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Chromatin Biology
Cell Cycle, DNA Replication & Repair
Chromatin Biology
This Anti-NuMA1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of NuMA1.
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected NuMA1 in a A431 cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected NuMA1 in a A431 cell lysate.
Target description
~ 238 kDa observed
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS, 50% glycerol, and 0.05% sodium azide.
Storage and Stability
Store at -20°C
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
WGK 2
Certificates of Analysis (COA)
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