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ABE1387

Sigma-Aldrich

Anti-Nuclear factor 1/C Antibody

serum, from rabbit

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Synonym(s):
Nuclear factor 1 C-type, NF1-C, CCAAT-box-binding transcription factor, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/C, NF-I/C, NFI-C, TGGCA-binding protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

technique(s)

ChIP: suitable
electrophoretic mobility shift assay: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NFIC(4782)

General description

Through evolution, eukaryotic cells have incorporated numerous viral promoter elements and incorporated them as part of their own gene regulatory elements. Nuclear factor 1/C was originally recognized as a transcription factor that bound the origin of replication for adenovirus type 2. Nuclear factor 1/C belongs to the CTF/NF-I family of eukaryotic DNA replication and transcription factors and is important in regulating the expression of many cellular developmental systems such as that of tooth development as well as multiple TGF-beta1 signaling mediated pathways systems.

Specificity

Recognizes multiple isoforms of Nuclear factor 1/C

Immunogen

Epitope: C-terminus
Recombinant protein corresponding to full length Human NFIC.

Application

Chromatin Immunoprecipitation Assay (ChIP): A representative lot of this antibody has been reported to work in ChIP. See Hebbar, P.B., and Archer, T.K. (2007), Vicent G.P. et al. (2010), and Brun, M., et al. (2013).

Electrophoretic Mobility Shift Assay (EMSA): A representative lot of this antibody has been reported to work in EMSA. See Johansson E.M., et al. (2003) and Kannius-Janson, M., et al. (2002).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
This Anti-Nuclear factor 1/C Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of Nuclear factor 1/C.

Quality

Evaluated by Western Blotting in U251 cell lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected NFIC isoforms at ~45-55 kDa in a U251 cell lysate.

Target description

~45-55 kDa observed

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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