Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

ABE257

Sigma-Aldrich

Anti-RAD51 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

DNA repair protein RAD51 homolog 1

Short name=HsRAD51

Short name=hRAD51

Alternative name(s):

RAD51 homolog A

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RAD51(5888)

General description

RAD51 is essential for homologous recombination of DNA during repair of double-strand breaks. The N-terminus of RAD51 functions to bind DNA and promote homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57, and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD51D). RAD51 is predominantly expressed in the testis and thymus but can also be found in the small intestine, placenta, colon, pancreas and ovaries.

Specificity

Other homologies: Bovine, Equine, and Canine (99% sequence homology).

Immunogen

Histidine-tagged recombinant protein corresponding to human RAD51.

Application

Anti-RAD51 Antibody is a Rabbit Polyclonal Antibody for detection of RAD51 also known as DNA repair protein RAD51 homolog 1, HsRAD51 & has been validated in WB, ICC & IP.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RAD51 in A431 and HeLa cells.

Immunoprecipitation Analysis: 40 µLof Anti-RAD51 immunoprecipitated RAD51 from HeLa RIPA lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:2,500 dilution of this antibody detected RAD51 on 10 µg of HeLa cell lysate.

Target description

~37 kDa observed. Two isoforms (isoform 1 at 37 kDa and isoform 2 at 26 kDa) produced as a result of alternative splicing are known to exist: .

Linkage

Replaces: MABE515

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Shiny Titus et al.
Science translational medicine, 5(172), 172ra21-172ra21 (2013-02-15)
The underlying mechanism behind age-induced wastage of the human ovarian follicle reserve is unknown. We identify impaired ATM (ataxia-telangiectasia mutated)-mediated DNA double-strand break (DSB) repair as a cause of aging in mouse and human oocytes. We show that DSBs accumulate
The role of DNA damage and repair in decitabine-mediated apoptosis in multiple myeloma.
Maes, K; De Smedt, E; Lemaire, M; De Raeve, H; Menu, E; Van Valckenborgh, E; McClue et al.
Oncotarget null
Zechen Yan et al.
Reproduction (Cambridge, England), 152(5), 431-437 (2016-08-06)
The production of haploid gametes by meiosis is a cornerstone of sexual reproduction and maintenance of genome integrity. Zfp38 mRNA is expressed in spermatocytes, indicating that transcription factor ZFP38 has the potential to regulate transcription during meiosis. In this study
Kaimin Guo et al.
Journal of cellular and molecular medicine, 22(10), 4863-4874 (2018-07-29)
Gametogenetin (GGN) binding protein 2 (GGNBP2) is a zinc finger protein expressed abundantly in spermatocytes and spermatids. We previously discovered that Ggnbp2 resection caused metamorphotic defects during spermatid differentiation and resulted in an absence of mature spermatozoa in mice. However
Franziska Foertsch et al.
Cell cycle (Georgetown, Tex.), 18(24), 3581-3588 (2019-11-17)
Knowledge about precise numbers of specific molecules is necessary for understanding and verification of biological pathways. The RAD51 protein is central in the repair of DNA double-strand breaks (DSBs) by homologous recombination repair and understanding its role in cellular pathways

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service