ABN421
Anti-Senataxin (OY11) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Probable helicase senataxin, Senataxin (OY11), Amyotrophic lateral sclerosis 4 protein, SEN1 homolog, Senataxin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SETX(23064)
General description
Probable helicase senataxin (UniProt Q7Z333; also known as Amyotrophic lateral sclerosis 4 protein, SEN1 homolog) is encoded by the SETX (also known as ALS4, AOA2, SCAR1, bA479K20.2) gene (Gene ID 23064) in human. Senataxin is implicated in both genome and RNA transcriptome homeostasis. Senataxin contains a helicase domain for mediating RNA interaction and an N-terminal domain for critical protein interactions. Senataxin is reported to colocalize with DNA repair proteins, including the DNA damage response protein 53BP1, at distinct nuclear foci in S/G2-phase human cells, and the number of these foci increases in response to impaired DNA replication or DNA damage. Senataxin is shown to resolve RNA/DNA hybrids (R loops) that arise during transcription, which can lead to genome instability unless they are resolved efficiently.
Immunogen
A mixture of four KLH-conjugated linear peptides corresponding to sequences from both N- and C-terminal halves of human senataxin.
Application
Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Senataxin from HeLa, U2OS, and HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin and siRNA-mediated Senataxin downregulation in HEK293 cells (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flag-tagged Senataxin in anti-Flag immunoprecipitates from transfected HEK293 cells expressing Flag-tagged Senataxin (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin in HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin and siRNA-mediated Senataxin downregulation in HEK293 cells (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flag-tagged Senataxin in anti-Flag immunoprecipitates from transfected HEK293 cells expressing Flag-tagged Senataxin (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin in HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
This Anti-Senataxin (OY11) Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Senataxin .
Quality
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Senataxin in 10 µg of HUVEC lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Senataxin in 10 µg of HUVEC lysate.
Target description
~300 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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