MilliporeSigma
All Photos(3)

Documents

ABN991

Sigma-Aldrich

Anti-phospho GLUT-1 Antibody (Ser226)

from rabbit, purified by affinity chromatography

Sign Into View Organizational & Contract Pricing

Synonym(s):
Solute carrier family 2, facilitated glucose transporter member 1, Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter, Human T-cell leukemia virus I and II receptor, Receptor for HTLV-1 and HTLV-2, phospho GLUT-1 (Ser226)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

horse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer226 )

Gene Information

human ... SLC2A1(6513)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4502803MABS132SAB4502804
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

technique(s)

immunocytochemistry: suitable, western blot: suitable, inhibition assay: suitable (peptide)

technique(s)

ELISA: 1:40000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:1000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

species reactivity

mouse, human

species reactivity

mouse, human, rat

species reactivity

human, rat

species reactivity

human

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Solute carrier family 2, facilitated glucose transporter member 1 (UniProt P11166; also known as Glucose transporter type 1 erythrocyte/brain, GLUT-1, HepG2 glucose transporter, Human T-cell leukemia virus I and II receptor, Receptor for HTLV-1 and HTLV-2) is encoded by the SLC2A1 (also known as DYT9, DYT17, DYT18, EIG12, GLUT, GLUT1, HTLVR, GLUT1DS, PED) gene (Gene ID 6513) in human. Glucose transporters (GLUT-1 to GLUT-7) constitute a family of integral membrane glycoproteins that facilitate cellular glucose uptake. In addition to mediating glucose uptake in adult tissues, GLUT-1 is the predominant glucose transporter in embryonic and fetal tissues. Two forms of GLUT-1 are found in human frontal cortex, the 55 kDa form of GLUT-1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT-1 predominantly regulates nonvascular glial glucose uptake.

Immunogen

Epitope: pSer226 in the fourth cytoplasmic domain (Loop 6).
KLH-conjugated linear peptide corresponding to a fourth cytoplasmic domain (Loop 6) sequence of human GLUT-1 with phosphorylated Ser226.

Application

Peptide Inhibition Analysis: 2.0 µg/mL from a representative lot detected Ser226 phosphorylated GLUT-1 in lysates from PMA-treated HeLa cells. Pre-incubation of the antibody with the immunogen peptide, but not the correspoding non-phosphorylated peptide, blocked the detection of the phospho GLUT-1 band.
Western Blotting Analysis: An 1:200 dilution (5 µg/mL) from a representative lot detected TPA-induced phosphorylation of wild-type GLUT-1, but not GLUT-1 with S226A mution in lysates from Rat2 fibroblasts expsssing the respective constructs via retrovirus-mediated transfection (Courtesy of Dr. Richard C. Wang, UT Southwestern Medical Center, Dallas, TX).
Western Blotting Analysis: An 1:200 dilution (5 µg/mL) from a representative lot detected a time-dependent GLUT-1 Ser226 phosphorylation induction in serum-starved human umbilical vein endothelial cells (HUVECs) and human aortic endothelial cells (HAECs) upon VEGF treatment (Courtesy of Dr. Richard C. Wang, UT Southwestern Medical Center, Dallas, TX).
Western Blotting Analysis: A representative lot detected PKC activation-induced GLUT-1 Ser226 phosphorylation in human aortic endothelial cells (HAECs) upon TPA (Cat. No. 500582 & 524400) treatment only the in the absence, but not in the presence, of PKC inhibitor Go 6983 (Cat. No. 365251) (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
Western Blotting Analysis: A representative lot detected a time-dependent GLUT-1 Ser226 phosphorylation induction in serum-starved human umbilical vein endothelial cells (HUVECs) upon VEGF or angiotensin II treatment (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
Western Blotting Analysis: A representative lot detected comparable Ser226 phosphorylation induction of exogenously expressed wild-type GLUT-1 or K526E mutant in transfected Rat2 fibroblasts upon PKC activator TPA (Cat. No. 500582 & 524400) treatment (Cat. No. 365251) (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
Western Blotting Analysis: A representative lot detected PKC activator TPA-induced GLUT-1 Ser226 phosphorylation in serum-starved HeLa, human primary cardiac endothelial cells, EA.hy926 human endothelial cells, and bEnd.3 mouse brain endothelial cells (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
Western Blotting Analysis: A representative lot detected PKC-catalyzed Ser226 phosphorylation of GST fusion protein containing wild-type GLUT-1 Loop 6 (4th cytoplasmic domain) seuqnce, but not GST-Loop 6 fusions with R223P, R223Q, R223W, or S226A mutation, in in vitro kinase assays (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
Western Blotting Analysis: A representative lot detected a time-dependent GLUT-1 Ser226 phosphorylation induction in serum-starved human aortic endothelial cells (HAECs) upon VEGF treatment (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
Immunocytochemistry Analysis: A representative lot detected PKC activation-induced GLUT-1 Ser226 phosphorylation in the membrane ruffles of human umbilical vein endothelial cells (HUVECs) upon TPA (Cat. No. 500582 & 524400) treatment (Lee, E.E., et al. (2015). Mol. Cell. 58(5):845-853).
Note: Process lysate samples by warming at 50°C for 10 minutes prior to gel loading. Avoid heating samples at a temperature higher than 60°C, which can cause aggregation of the target protein.
This Anti-phospho GLUT-1 Antibody (Ser226) is validated for use in Western Blotting, Peptide Inhibition Assay, Immunocytochemistry for the detection of phospho GLUT-1.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Ser226 phosphorylated GLUT-1 in lysates from PMA-treated HeLa cells.

Target description

~50 kDa observed. A smeared banding pattern between 45-75 kDa can often be seen, representing target bands with varying degrees of glycosylation.

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Alexandra M Kraft et al.
American journal of physiology. Cell physiology, 319(5), C910-C921 (2020-09-10)
Some patients treated for ductal carcinoma in situ (DCIS) of the breast will experience cancer recurrences, whereas other patients will not. Unfortunately, current techniques cannot identify which preinvasive lesions will lead to recurrent cancer. Because the mechanism of cancer recurrence
Eunice E Lee et al.
Methods in molecular biology (Clifton, N.J.), 1713, 57-67 (2017-12-09)
Understanding the physiological regulation of glucose transport requires the analysis of transporters, like GLUT1, in diverse tissue types. We document the utility of viral vectors for the stable expression of wild-type and modified GLUT1 transporter in different types of mammalian
De-Dong Li et al.
Cell host & microbe, 30(4), 530-544 (2022-03-23)
Combating fungal pathogens poses metabolic challenges for neutrophils, key innate cells in anti-Candida albicans immunity, yet how host-pathogen interactions cause remodeling of the neutrophil metabolism is unclear. We show that neutrophils mediate renal immunity to disseminated candidiasis by upregulating glucose
Zhenxing Zhang et al.
Nature communications, 12(1), 5872-5872 (2021-10-09)
Glucose transporter GLUT1 is a transmembrane protein responsible for the uptake of glucose into the cells of many tissues through facilitative diffusion. Plasma membrane (PM) localization is essential for glucose uptake by GLUT1. However, the mechanism underlying GLUT1 PM localization
Katharina Timper et al.
Cell metabolism, 31(6), 1189-1205 (2020-05-21)
Astrocytes represent central regulators of brain glucose metabolism and neuronal function. They have recently been shown to adapt their function in response to alterations in nutritional state through responding to the energy state-sensing hormones leptin and insulin. Here, we demonstrate

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service