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Key Documents

ABS1624

Sigma-Aldrich

Anti-Lunapark Antibody

serum, from rabbit

Synonym(s):

Protein lunapark, Ulnaless

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

monkey, human

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

General description

Protein lunapark (UniProt Q9C0E8; also known as Ulnaless) is encoded by the LNP (also known as KIAA1715) gene (Gene ID 80856) in human. The endoplasmic reticulum (ER) consists of a polygonal network of sheets and tubules interconnected by three-way junctions that undergoes continual remodeling through two competing processes. The branching and fusion of tubules lead to the formation of new three-way junctions and polygons, while junction sliding and ring closure lead to polygon loss. Both yeast Lnp1 and mammalian lunapark (mLnp1) are reported to play an important role in the maintenance of ER morphology through junctions stabilization. Study conducted in COS-7 cells demonstrated that junctions with lunapark are less mobile than junctions lacking lunapark. New junctions acquired lunapark tend to remain stable within the ER network, whereas the nascent junctions that fail to acquire lunapark preferentially undergo ring closure.

Specificity

Exprected to react with all four spliced isoforms of human lunapark reported by UniProt (Q9C0E8).

Immunogen

GST-tagged full-length recombinant human Lunapark.

Application

Detect Lunapark using this Anti-Lunapark Antibody validated for use in Western Blotting.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Western Blotting Analysis: The purified antibody was used to detect Lunapark expression in COS-7 cells and confirm siRNA-medidated Lunapark downregulation (Chen, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(2):418-423).

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Lunapark in 10 Āµg of HeLa cell lysate.

Target description

~47 kDa observed. 47.74 kDa (isoform 1), 47.09 kDa (isoform 2), 33.01 kDa (isoform 3), and 50.85 kDa (isoform 4) calculated.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20Ā°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20Ā°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Hiroaki Kajiho et al.
Scientific reports, 9(1), 13152-13152 (2019-09-13)
Endoplasmic reticulum (ER) tubules connect each other by three-way junctions, resulting in a tubular ER network. Oligomerization of three-way junction protein lunapark (Lnp) is important for its localization and the three-way junction stability. On the other hand, Lnp has an

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