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ABS82-AF647

Sigma-Aldrich

Anti-VEGF Antibody, Alexa Fluor 647 Conjugate

from rabbit, ALEXA FLUOR 647

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Synonym(s):
Anti-MVCD1, Anti-VEGF, Anti-VPF, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, Alexa Fluor 647 Conjugate
eCl@ss:
32160702
NACRES:
NA.45

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 647

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

nonhuman primates (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VEGFA(7422)

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This Item
07-1420AB1876-IABS82
vibrant-m

07-1420

Anti-VEGF Antibody

vibrant-m

AB1876-I

Anti-VEGF-A Antibody

vibrant-m

ABS82

Anti-VEGF Antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

ALEXA FLUOR 647

conjugate

-

conjugate

-

conjugate

-

Gene Information

human ... VEGFA(7422)

Gene Information

human ... VEGFA(7422)

Gene Information

mouse ... Vegfa(22339)
rat ... Vegfa(83785)

Gene Information

human ... VEGFA(7422)

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

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General description

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively). All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and also plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.

Specificity

Other homologies: Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology).
This antibody recognizes VEGF at the N-terminus.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in HeLa cells.

Target description

~55 kDa observed

Physical form

Affinity purified
Purified rabbit antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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