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Key Documents

ABT58

Sigma-Aldrich

Anti-phospho PKA-RII (Ser96) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

PRKAR2A, protein kinase, cAMP-dependent, regulatory, type II, alpha

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer96)

Gene Information

human ... PRKAR2A(5576)

General description

cAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.

Specificity

The antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.

Immunogen

Epitope: Phosphorylated Ser96 of PKA-RIIalpha
KLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.

Application

Research Category
Cell Structure

Signaling
Research Sub Category
Cytoskeletal Signaling

Kinases & Phosphatases
This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).

Quality

Evaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.

Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.

Target description

~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.

Linkage

Replaces: 06-704

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Forskolin untreated and treated PC12 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L Zhou et al.
Translational psychiatry, 6(9), e881-e881 (2016-09-07)
Intracellular cAMP and serotonin are important modulators of anxiety and depression. Fluoxetine, a selective serotonin reuptake inhibitor (SSRI) also known as Prozac, is widely used against depression, potentially by activating cAMP response element-binding protein (CREB) and increasing brain-derived neurotrophic factor

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