ACS500024
Amicon® Pro Purification System
sample volume 10 mL, polypropylene tube (for collection)
Synonym(s):
Amicon Ultra-0.5 adapter
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About This Item
material
polyethylene cap
polyethylene frit
polypropylene device (for exchange)
polypropylene frit
polypropylene tube (for collection)
polypropylene tube (holder)
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 ea
manufacturer/tradename
Amicon® Pro
parameter
10 mL sample volume
technique(s)
buffer exchange: suitable
protein purification: suitable (antibody labeling)
protein purification: suitable (concentration)
protein purification: suitable (enrichment)
L
12 cm (4.7 in.)
diam.
2.97 cm
working volume
10 mL
shipped in
ambient
General description
Features and Benefits
Physical form
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
Protocols
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Related Content
E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.
Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.
bios-C-epdf-1043-1304-SP
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