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AG220

Sigma-Aldrich

Choline Acetyltransferase, for Blocking AB143, AB144P Antibodies

Synonym(s):

ChAT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody product type

primary antibodies

form

liquid

manufacturer/tradename

Chemicon®

concentration

0.25 mg/mL

technique(s)

cell based assay: suitable

UniProt accession no.

shipped in

dry ice

General description

Recombinant rat ChAT

Application

For use in blocking the staining of Chemicon′s catalog numbers AB143, AB144P and AB144. Suggested concentration 2-5 μg/mL. AG220 should be incubated with the antibody for 3 hours at +2-8°C prior to being used on the tissue sections.

Enzyme is denatured by heat, but its activity level has not been determined. Residual activity may be present.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Physical form

Liquid in 50 mM KPO4 buffer pH 7.5 containing 10% glycerol and 0.05M NaCl. Contains no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nuclear organization of cholinergic, putative catecholaminergic and serotonergic nuclei in the brain of the eastern rock elephant shrew, Elephantulus myurus.
Raymond P Pieters,Nadine Gravett,Kjell Fuxe,Paul R Manger
Journal of Chemical Neuroanatomy null
Sara Morcuende et al.
The Journal of comparative neurology, 519(12), 2335-2352 (2011-04-02)
Neurotrophins acting through high-affinity tyrosine kinase receptors (trkA, trkB, and trkC) play a crucial role in regulating survival and maintenance of specific neuronal functions after injury. Adult motoneurons supplying extraocular muscles survive after disconnection from the target, but suffer dramatic
Effect of treatment with the cholinesterase inhibitor rivastigmine on vesicular acetylcholine transporter and choline acetyltransferase in rat brain.
Seyed Khosrow Tayebati,Maria Antonietta Di Tullio,Francesco Amenta
Clinical and Experimental Hypertension (1993)
Variability in the occurrence of nitric oxide synthase immunoreactivity in different populations of rat sympathetic preganglionic neurons.
Julia M Hinrichs,Ida J Llewellyn-Smith
The Journal of Comparative Neurology null
Nuclear organization of cholinergic, putative catecholaminergic and serotonergic systems in the brains of two megachiropteran species.
Dell, Leigh-Anne, et al.
Journal of Chemical Neuroanatomy, 40, 177-195 (2010)

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