AP500P
Goat Anti-Mouse IgM µ chain Antibody, HRP conjugate, Species Adsorbed
Chemicon®, from goat
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
IgM constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.
Specificity
Reacts with heavy chain of mouse IgM. Absorbed for mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.
The antibody reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Immunogen
Epitope: IgM µ chain
Prepared from purified mouse IgM µ chain. Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.
Application
Detect Mouse IgM µ chain using this Goat anti-Mouse IgM µ chain Antibody, HRP conjugate, Species Adsorbed validated for use in ELISA.
ELISA: 1:4,000-1:8,000.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Fragment Specific Secondary Antibodies
Secondary Antibodies Adsorbed for Dual Labeling
Fragment Specific Secondary Antibodies
Physical form
Affinity Chromatography
Purified by affinity chromatography on mouse IgM covalently linked to agarose. Liquid in 50% glycerol/50% PBS, pH 7.4.
Storage and Stability
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
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