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CBA098

Sigma-Aldrich

InnoCyte Monocyte Cell Migration Assay

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usage

sufficient for 96 tests

Quality Level

packaging

pkg of 1 96-well plate(s)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

input

sample type monocyte(s)

detection method

fluorometric

shipped in

wet ice

General description

The InnoCyte Monoctye Cell Migration Assay is designed to study the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines.

Components

96-Well Monocyte Cell Migration Chamber, Black, Conical 96-Well Plate, D-PBS, Calcein-AM, and a user protocol.

Warning

Toxicity: Irritant (B)

Principle

The Calbiochem InnoCyte 96-Well Monoctye Cell Migration Assay is intended for the study of the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines. To study these cell types, please use Cat. No. CBA010 or CBA017.

Preparation Note

Cell Labeling Mixture: Warm the D-PBS and Calcein-AM Solution to room temperature. Add 35 µl Calcein-AM Solution to 10 ml D-PBS. Mix well. Note: Prepare just before use (see step 7 of the Detailed Protocol below).

Storage and Stability

Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.

Other Notes

Cravens, P.D. 2007. Scan. J. Immunol.65, 514.
Charo, I.F., et al. 2006. N. Engl. J. Med.354, 610.
Knitscher, U., et al. 2000. Eur. J. Pharmacol.401, 259.
Turner, L., et al. 1999. Eur. J. Immunol.29, 2288.
Cross, A.K., et al. 1997. Cytokine9, 521.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13


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