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CBL496-I

Sigma-Aldrich

Anti-CD34 Antibody, clone QBEnd/10

clone QBEnd/10, from mouse

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Synonym(s):
Hematopoietic progenitor cell antigen CD34
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

QBEnd/10, monoclonal

species reactivity

human, monkey

packaging

antibody small pack of 25 μL

technique(s)

electron microscopy: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1λ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... CD34(947)

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SAB4701114MABF175EMABF173E
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

clone

QBEnd/10, monoclonal

clone

581, monoclonal

clone

UCHT1, monoclonal

clone

SK7, monoclonal

species reactivity

human, monkey

species reactivity

-

species reactivity

human

species reactivity

human

packaging

antibody small pack of 25 μL

packaging

-

packaging

-

packaging

-

technique(s)

electron microscopy: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

flow cytometry: 10 μL

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

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General description

Hematopoietic progenitor cell antigen CD34 (UniProt: P28906; also known as CD34) is encoded by the CD34 gene (Gene ID: 947) in human. CD34 is a highly glycosylated single-pass type I membrane protein that is expressed on hematopoietic progenitor cells and small vessel endothelium of a variety of tissues. Under normal conditions, CD34+ expressing cells account for about 1 2% of the total bone marrow cells. It serves as an adhesion molecule that plays a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It is also reported to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is synthesized with a signal peptide (aa 1-31) that is cleaved off in the mature form. The mature form has an extracellular domain (aa 32-290), a transmembrane domain (aa 291-311), and a cytoplasmic domain (aa 312-385). Two isoforms of CD34 have been described that are produced by alternative splicing.

Specificity

Clone QBEnd/10 specifically detects CD34 in human and non-human primates.

Immunogen

Human placental endothelial membrane vesicles.

Application

Anti-CD34, clone QBEnd/10, Cat. No. CBL496-I, is a mouse monoclonal antibody that detects CD34 and has been tested for use in Electron Microscopy, Flow Cytometry, Immunofluorescence and Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD34 in human brain tissue sections.

Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot was used to sort CD34+ cells from bone marrow. (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26).

Immunofluorescence Analysis: A representative lot detected CD34 in Immunofluorescence applications (Miki, T., et. al. (2010). Mol Cancer Res. 8(5):665-76).

Electron Microscopy Analysis: A representative lot detected CD34 in Electron Microscopy applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26).

Immunohistochemistry Analysis: A representative lot detected CD34 in Immunohistochemistry applications (Engler, J.R., et. al. (2012). PLoS One. 7(8):e43339; Fina, L., et. al. (1990). Blood. 75(12):2417-26).

Flow Cytometry Analysis: A representative lot detected CD34 in Flow Cytometry applications (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26; Fina, L., et. al. (1990). Blood. 75(12):2417-26).

Western Blotting Analysis: A representative lot detected CD34 in Western Blotting applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26).

Quality

Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected CD34 in human kidney tissue sections.

Target description

40.72 kDa Calculated. This antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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