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ECM541

Sigma-Aldrich

ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)

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eCl@ss:
32161000

technique(s)

cell based assay: suitable

Quality Level

input

sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)

detection method

colorimetric

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ECM540ECM545ECM531
input

sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)

input

-

input

-

input

-

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric

detection method

colorimetric

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. In cases where optimal cell growth conditions are not well defined and critical adhesion protein requirements are unknown, identifying the ideal ECM protein(s) at functionally relevant concentration(s) can be a time-consuming and labor-intensive process. The 96-well, colorimetric ECM Cell Culture Optimization Array provides a multiparametric assay that allows researchers to: (1) quickly identify ECM(s) that best promote cell-type specific adhesion and (2) pinpoint the optimal ECM concentration required to promote maximum adhesion for a particular cell type.

How does the ECM Cell Culture Optimization Array work?
The foundation of our kit is a 96-well microtiter plate containing several commonly-used ECM proteins. Collagen I, laminin, fibronectin, and vitronectin are arrayed in triplicate with concentrations ranging from 0.125 μg/mL to 20 μg/mL. You simply culture your cells on the plate, and use the included reagents to block, stain, and analyze the adhesion capacity with a plate reader. We have extensively tested and optimized this kit on a variety of cell types including HEK293 cells, and human and rodent neural stem cells.

Application

Research Category
Cell Structure
The ECM Cell Culture Optimization Array is the first commercially-available tool of its kind to enable researchers to not only quickly identify the best ECM protein for their cell culture environment, but also pinpoint the concentration needed to achieve optimal cell growth conditions.
The ECM cell culture Optimization Array enables researchers to identify the best ECM protein for their cell culture environment & also pinpoint the concentration needed to achieve optimal cell growth conditions.

Packaging

1 plate, 96-wells

Components

  • ECM Gradient Array Plate: One 96-well plate with 12 strips
  • 30%BSA: (Part No. CS201638) One vial - 0.5mL
  • Cell Stain Solution: (Part No. 90144) One bottle - 20 mL.
  • Extraction Buffer: (Part No. 90145) One bottle - 20 mL
  • Assay Buffer: (Part No. 2003646) One bottle - 100 mL

Storage and Stability

The ECM Gradient Array Plate should be stored in the foil pouch at -70ºC, and used within three months of the date of receipt. The remaining kit components should be stored at 2º to 8ºC.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


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