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Sigma-Aldrich

EmbryoMax® Electroporation Buffer

The EmbryoMax Electroporation buffer is used to dissolve the DNA that is to be transfected into mouse embryonic stem cells.

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eCl@ss:
32160801
NACRES:
NA.75

Quality Level

form

liquid

manufacturer/tradename

Specialty Media
EmbryoMax®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

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This Item
1.06177T928511417959001
Millipore

Millipore

1.06177

TBE buffer

SuRE/Cut™ Buffer A pH 8.1-8.3 (37 °C), suitable for molecular cloning

Roche

11417959001

SuRE/Cut Buffer A

technique(s)

cell culture | stem cell: suitable

technique(s)

-

technique(s)

-

technique(s)

molecular cloning: suitable

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

-

manufacturer/tradename

Specialty Media

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

-

input

-

input

-

General description

This Electroporation buffer is used to dissolve the DNA that is to be transfected into the ES cells. Generally 25-40μg of the knockout construct DNA is dissolved in 30μl of the buffer.

Storage and Stability

Store at 2-8°C upto 18 months.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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