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FCABS325PE

Sigma-Aldrich

Milli-Mark® Anti-acetyl-Histone H3-PE Antibody

Milli-Mark®, from rabbit

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Synonym(s):
H3t, H3/t, H3/g, Histone H3.1t
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

PE

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Tetrahymena sp., human, mouse

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

acetylation (Lys9,Lys14)

Gene Information

human ... H3F3B(3021)

Related Categories

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.

Specificity

Antibody recognizes Tetrahymena acetyl Histone H3
This sequence is highly conserved and predicted to have broad species crossreactivity.

Immunogen

KLH-conjugated peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 (ARTKQTAR[K*]STGG[K*]APRKQLC) where K* is acetylated

Application

This Milli-Mark Anti-acetyl-Histone H3-PE Antibody is validated for use in FC for the detection of acetyl-Histone H3.

Quality

Evaluated by flow cytometry using HeLa cells

Target description

15.5 kDa Calculated

Physical form

Purified rabbit polyclonal IgG conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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