HTMP2MAG-54K
MILLIPLEX® Human TIMP Magnetic Bead Panel 2 - Immunology Multiplex Assay
TIMP Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple TIMP biomarkers in human cell/tissue culture supernatant samples.
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
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Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 77-95%
standard curve range: 10-10,000 pg/mL
(TIMP-4)
standard curve range: 20-20,000 pg/mL
(TIMP-1)
standard curve range: 49-50,000 pg/mL
(TIMP-2)
standard curve range: 98-100,000 pg/mL
(TIMP-3)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
Tissue Inhibitors of Metalloproteinases (TIMPs) comprise a family of four inhibitors known as TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Each member of the TIMP family has its own distinct profile of metalloproteinase inhibition. The matrix metalloproteinases (MMPs) are a family of zinc proteases involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, tissue and bone remodeling, wound healing, tissue morphogenesis, angiogenesis, and tumor metastasis. MMPs are also known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands (such as the FAS ligand), cell proliferation, differentiation, and chemokine in/activation. The normal function of MMPs must be precisely regulated by their endogenous protein inhibitors, TIMPs . TIMPs inhibit MMPs by forming 1:1, non-covalent complexes with MMPs, thereby blocking access of substrates to the MMP catalytic site. Except for inhibition of active MMPs, TIMPs also exhibit several other biochemical and physiological/biological functions such as proMMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Disruption of the MMP/TIMP balance can result in serious diseases such as arthritis, cardiovascular disorders, tumor growth and metastasis.
The MILLIPLEX® Human TIMP Panel 2 Bead kit (TIMP-1, -2, -3, -4) has been designed for tissue/cell culture samples.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
The MILLIPLEX® Human TIMP Panel 2 Bead kit (TIMP-1, -2, -3, -4) has been designed for tissue/cell culture samples.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
Specificity
Cross Reactivty
No significant cross-reactivity was observed among analytes in this panel.
No significant cross-reactivity was observed among analytes in this panel.
Application
- Analytes: TIMP-1, TIMP-2, TIMP-3, TIMP-4
- Recommended Sample type: Tissue and cell culture supernatants
- Assay Run Time: One day or overnight
- Research Category: Inflammation & Immunology
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Other Notes
Sensitivity: Refer to kit protocol for sensitivities of individual analytes.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Larissa Berloffa Belardin et al.
Scientific reports, 9(1), 903-903 (2019-01-31)
Matrix Metalloproteinases (MMPs) and their regulators - Tissue Inhibitors of Matrix Metalloproteinases (TIMPs) - participate in extracellular matrix remodeling, fibrosis, and semen liquefaction, as well as to inflammatory activity. Seminal plasma has been shown to contain MMPs (MMP-2 and MMP-9)
Abstracts from the 43rd American Society of Andrology Annual Meeting, 21-24 April 2018, Portland, Oregon.
Andrology, 6 Suppl 1, 1-98 (2018-03-28)
Jonathan H Soslow et al.
Journal of cardiac failure, 25(4), 259-267 (2019-02-15)
Cardiomyopathy is the leading cause of death in Duchenne muscular dystrophy (DMD). Standard cardiac biomarkers are poor indicators of DMD cardiovascular disease. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) regulate collagen turnover. Given the cardiac fibrosis seen in
Isoproterenol effects evaluated in heart slices of human and rat in comparison to rat heart in vivo.
Julia E Herrmann et al.
Toxicology and applied pharmacology, 274(2), 302-312 (2013-11-30)
Human response to isoproterenol induced cardiac injury was evaluated by gene and protein pathway changes in human heart slices, and compared to rat heart slices and rat heart in vivo. Isoproterenol (10 and 100μM) altered human and rat heart slice
Preethi Vijayaraj et al.
Cell reports, 29(11), 3488-3505 (2019-12-12)
Progressive organ fibrosis accounts for one-third of all deaths worldwide, yet preclinical models that mimic the complex, progressive nature of the disease are lacking, and hence, there are no curative therapies. Progressive fibrosis across organs shares common cellular and molecular
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