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MAB10754

Sigma-Aldrich

Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1

clone 2B12.1, from mouse

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Synonym(s):
caspase 8, apoptosis-related cysteine peptidase, caspase-8, caspase 8, apoptosis-related cysteine protease, MACH-beta-1/2/3/4 protein, Apoptotic protease Mch-5, Apoptotic cysteine protease, MACH-alpha-1/2/3 protein, FADD-homologous ICE/ced-3-like proteas
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2B12.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CASP8(841)

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SAB5700197HPA005688SAB56000344
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

clone

2B12.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

RM442, monoclonal

Gene Information

human ... CASP8(841)

Gene Information

human ... CASP8(841)

Gene Information

human ... CASP8(841)

Gene Information

human ... CASP8(841)

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

-

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:50-1:200

technique(s)

-

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General description

Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death. Caspase-8 is recruited to death-inducing signaling complex (DISC), CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18. Active caspase-8 or the p18 subunit, is the first in the caspase signaling cascade, and it signals downstream caspases to eventually induce apoptosis.

Specificity

This antibody recognizes the active form (p18 subunit) of Caspase-8.

Immunogen

KLH-conjugated linear peptide corresponding to the active form of human Caspase-8.

Application

Detect Caspase-8 (active form p18 subunit) using this Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1 validated for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected active Caspase-8 (p18 subunit) in etoposide-treated HeLa cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Caspases

Apoptosis - Additional

Quality

Evaluated by Western Blot in camptothecin-treated and untreated Jurkat lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected the active form of Caspase-8 (p18 subunit) in 10 µg of camptothecin-treated Jurkat lysates. Untreated jurkat lysate exhibited no detection of active Caspase-8.

Target description

~18 kDa observed

Linkage

Replaces: 04-573; 04-574

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Camptothecin-treated and untreated Jurkat lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mari Kamiya et al.
Nature communications, 13(1), 166-166 (2022-01-12)
Muscle cell death in polymyositis is induced by CD8+ cytotoxic T lymphocytes. We hypothesized that the injured muscle fibers release pro-inflammatory molecules, which would further accelerate CD8+ cytotoxic T lymphocytes-induced muscle injury, and inhibition of the cell death of muscle
Paolo Petazzi et al.
Neurobiology of disease, 67, 49-56 (2014-03-25)
Epigenetic mechanisms are fundamental for shaping the activity of the central nervous system (CNS). Methyl-CpG binding protein 2 (MECP2) acts as a bridge between methylated DNA and transcriptional effectors responsible for differentiation programs in neurons. The importance of MECP2 dosage

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