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Key Documents

MAB1956Z

Sigma-Aldrich

Anti-Integrin α5 Antibody, clone P1D6, azide free

clone P1D6, Chemicon®, from mouse

Synonym(s):

CD49e, MAB1956

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

P1D6, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

isotype

IgG3

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGA5(3678)

General description

Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling.

Specificity

Reacts with Human alpha 5 integrin. Reactivity with other species is unknown.

Application

MAB1956Z is suitable for use in attachment inhibition assays using fibroblasts, endothelial cells, platelets and T & B lymphocytes on fibronectin.

MAB1956Z is not reactive with the denatured alpha 5 subunit. For immunoblotting, a dot blot is suggested as an alternative to western blotting.
Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin α5 Antibody, clone P1D6, azide free is validated for use in IH, FUNC for the detection of Integrin α5.

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal in 0.01M Phosphate, 150mM NaCl, pH 7.4, containing no preservatives.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Widely expressed, ovary

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Manish K Rana et al.
Journal of cell science, 128(6), 1083-1089 (2015-01-27)
Increased production and assembly of extracellular matrix proteins during transdifferentiation of epithelial cells to a mesenchymal phenotype contributes to diseases such as renal and pulmonary fibrosis. TGF-β and hypoxia, two cues that initiate injury-induced fibrosis, caused human kidney cells to
John J Grzesiak et al.
Surgery, 141(6), 804-814 (2007-06-15)
We have shown recently that alpha(2)beta(1) integrin-mediated type I collagen adhesion promotes a more malignant phenotype in pancreatic cancer cell lines than other extracellular matrix (ECM) proteins. MiaPaCa-2 cells, by contrast, do not express collagen-binding integrins, but are metastatic in
Juan Pablo Robles et al.
The Journal of biological chemistry, 298(3), 101695-101695 (2022-02-11)
Vascular endothelial cells (ECs) form a critical interface between blood and tissues that maintains whole-body homeostasis. In COVID-19, disruption of the EC barrier results in edema, vascular inflammation, and coagulation, hallmarks of this severe disease. However, the mechanisms by which
Meng Lu et al.
Hepatology (Baltimore, Md.), 68(1), 317-332 (2018-01-23)
Hepatocytes are epithelial cells with highly specialized polarity. The disorder and loss of hepatocyte polarity leads to a weakness of cell adhesion and connection, the induction of epithelial-mesenchymal transition, and eventually the occurrence of hepatocellular carcinoma (HCC). Cluster of differentiation
Pascale Desjardins et al.
Journal of cellular physiology, 237(5), 2434-2450 (2022-02-13)
Due to its superficial anatomical localization, the cornea is continuously subjected to injuries. Damages to the corneal epithelium trigger important changes in the composition of the extracellular matrix to which the basal human corneal epithelial cells (hCECs) attach. These changes

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