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MAB1998Z

Sigma-Aldrich

Anti-Integrin α2β1 Antibody, clone BHA2.1, azide free

clone BHA2.1, Chemicon®, from mouse

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Synonym(s):
VLA-2, MAB1998
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BHA2.1, monoclonal

species reactivity

human, pig

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGA2(3673)

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This Item
MAB1998MAB1950ZMAB1950
Gene Information

human ... ITGA2(3673)

Gene Information

human ... ITGA2(3673)

Gene Information

human ... ITGA2(3673)

Gene Information

human ... ITGA2(3673)

species reactivity

human, pig

species reactivity

human, canine, pig

species reactivity

human

species reactivity

human

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

BHA2.1, monoclonal

clone

BHA2.1, monoclonal

clone

P1E6, monoclonal

clone

P1E6, monoclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

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Specificity

Reacts with the collagen receptor VLA-2 (alpha2beta1) integrin complex.

Application

Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin α2β1 Antibody, clone BHA2.1, azide free is validated for use in FC, IH, IP, FUNC for the detection of Integrin α2β1.
flow cytometry: 10 μg/mL

Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1

immunohistochemistry in frozen tissue sections: 5 μg/mL

Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.

Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.

Final working dilutions must be determined by end user.

Physical form

Format: Purified
Protein A purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing no preservative.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Skin, CD4 & CD8 positive T cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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