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Key Documents

MAB3582

Sigma-Aldrich

Anti-Fascin Antibody, clone 55K2

clone 55K2, Chemicon®, from mouse

Synonym(s):

Anti-FAN1, Anti-SNL, Anti-p55

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

55K2, monoclonal

species reactivity

echinoderm, sea urchin, gerbil, human

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

suitability

not suitable for immunoprecipitation

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FSCN1(6624)

Specificity

Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.

Immunogen

Human fascin

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.

Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.

Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.

Analysis Note

Control
POSITIVE CONTROL:

Hela cells, neuronal tissues, antigen presenting dendritic cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Min Zhang et al.
OncoTargets and therapy, 9, 1123-1133 (2016-04-05)
It has been previously reported that the deregulation of microRNAs in gastric cancer (GC) was correlated with the progression and prognosis. miR-429, a member of the miR-200 family, was previously shown to play an important role in human carcinomas. Our
Heath E Johnson et al.
The Journal of cell biology, 208(4), 443-455 (2015-02-11)
Mesenchymal cells such as fibroblasts are weakly polarized and reorient directionality by a lamellipodial branching mechanism that is stabilized by phosphoinositide 3-kinase (PI3K) signaling. However, the mechanisms by which new lamellipodia are initiated and directed are unknown. Using total internal
Protein expression dynamics during postnatal mouse brain development.
Laeremans, A; Van de Plas, B; Clerens, S; Van den Bergh, G; Arckens, L; Hu, TT
Journal of experimental neuroscience null
Thomas A Masters et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(7), 1595-1600 (2017-02-02)
Myosin VI (MYO6) is the only myosin known to move toward the minus end of actin filaments. It has roles in numerous cellular processes, including maintenance of stereocilia structure, endocytosis, and autophagosome maturation. However, the functional necessity of minus-end-directed movement
S Al-Saad et al.
British journal of cancer, 99(9), 1476-1483 (2008-10-16)
Vimentin, nuclear factor-kappaB (NF-kappaB) p105, fascin, E-cadherin, TGF-beta, Par6 and atypical PKC are molecular markers that play an important role in cell differentiation. Herein, we investigate their prognostic impact in primary non-small-cell carcinoma (NSCLC). Tumour tissue samples from 335 resected

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