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MAB4301

Sigma-Aldrich

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480

clone MC-480, Chemicon®, from mouse

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Synonym(s):
SSEA-1, CD15, Lewis X, 3-FAL
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

MC-480, monoclonal

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)

isotype

IgM

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... FUT4(2526)

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This Item
FCMAB117PMAB4301XFCMAB116P
species reactivity

mouse, human, rat

species reactivity

rat, mouse, human

species reactivity

mouse, human, rat

species reactivity

human

clone

MC-480, monoclonal

clone

MC-480, monoclonal

clone

MC-480, monoclonal

clone

MC-813-70, monoclonal

Gene Information

human ... FUT4(2526)

Gene Information

human ... FUT4(2526)

Gene Information

human ... FUT4(2526)

Gene Information

human ... FUT4(2526)

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

packaging

-

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General description

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Specificity

This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Immunogen

F9 tetracarcinoma stem cells (X-irradiated).

Application

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
Immunohistochemistry:
A previous lot of this antibody was tested on IH.

Immunoprecipitation:
A previous lot of this antibody was tested on IP.

Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.

Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.

Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.

Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Neural Stem Cells

Quality

Tested

Target description

220 kDa

Physical form

Format: Purified
Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
P19 mouse embryonic carcinoma cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Efficient generation of iPS cells from skeletal muscle stem cells.
Tan, KY; Eminli, S; Hettmer, S; Hochedlinger, K; Wagers, AJ
Testing null
Hans M Larsson et al.
PloS one, 7(11), e49874-e49874 (2012-12-05)
While cell sorting usually relies on cell-surface protein markers, molecular beacons (MBs) offer the potential to sort cells based on the presence of any expressed mRNA and in principle could be extremely useful to sort rare cell populations from primary
Haiyang Zhang et al.
Urology, 79(3), 746-746 (2011-12-27)
To identify potential stem cells in urinary bladder by label-retaining cell (LRC) strategy and immunostaining for putative stem cell markers. Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders
Nicolas Christoforou et al.
PloS one, 8(6), e65963-e65963 (2013-06-21)
The mammalian heart has little capacity to regenerate, and following injury the myocardium is replaced by non-contractile scar tissue. Consequently, increased wall stress and workload on the remaining myocardium leads to chamber dilation, dysfunction, and heart failure. Cell-based therapy with
Yan-Min Feng et al.
Cell cycle (Georgetown, Tex.), 13(5), 782-791 (2014-01-09)
A critical process of early oogenesis is the entry of mitotic oogonia into meiosis, a cell cycle switch regulated by a complex gene regulatory network. Although Notch pathway is involved in numerous important aspects of oogenesis in invertebrate species, whether

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