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MAB4418

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Anti-Achaete Scute homolog 2 Antibody, clone 7E2

clone 7E2, from mouse

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Synonym(s):
achaete-scute complex (Drosophila) homolog-like 2, achaete-scute complex homolog 2 (Drosophila), achaete-scute complex homolog-like 2, achaete-scute complex-like 2, achaete-scute complex-like 2 (Drosophila), mammalian achaete/scute homologue 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7E2, monoclonal

species reactivity

human

technique(s)

ChIP: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ASCL2(430)

General description

Achaete Scute homolog 2 (also known as mammalian aschaete-scute homolog 2 or Mash2) is a nuclear protein expressed specifically in the extravillous trophoblasts of developing placenta. Placental development involves control by the basic helix-loop-helix transcription factor Achaete Scute homolog 2, which may regulate HIF (hypoxia) in the formation of spongiotrophoblasts. Targeted mutagenesis of Achaete Scute homolog 2 yielded loss of function results in embryonic lethality at midgestation due to placental failure associated with a lack of spongiotrophoblasts and reduced labyrinthine trophoblast layers. In addition to healthy placental development, Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. Achaete Scute homolog 2 has also been implicated as a controller of intestinal stem cell fate and is essential for the maintenance of adult intestinal stem cells (Tanaka, M., et al., (1997) Dev Biol. 190(10):55-65).

Specificity

This antibody recognizes Achaete Scute homolog 2 near the C-terminus.

Immunogen

His-tagged recombinant protein corresponding to human Achaete Scute homolog 2 near the C-terminus.

Application

Anti-Achaete Scute homolog 2 Antibody, clone 7E2 detects level of Achaete Scute homolog 2 & has been published & validated for use in WB, IC, ChIP.
Immunocytochemmistry Analysis: A previous lot was used by an independent laboratory in IC (Van de Flier, L.G., et al., 2009)

Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChIP (Hatzis, P., et al., 2008).

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Achaete Scute homolog 2 in 10 µg of human placenta tissue lysate.

Target description

~20 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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