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Key Documents

MAB5326C3

Sigma-Aldrich

Anti-Nestin Antibody, clone 10C2, Cy3 conjugate

clone 10C2, from mouse, CY3 conjugate

Synonym(s):

Nestin10c2, NES, Nbla00170, FLJ21841

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

CY3 conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

10C2, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NP_006608

UniProt accession no.

P48681

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NES(10763)

General description

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

Specificity

This antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.

Immunogen

Fusion protein corresponding to human Nestin.

Application

Detect Nestin using this Anti-Nestin Antibody, clone 10C2, Cy3 conjugate validated for use in ICC.
Research Category
Stem Cell Research

Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Quality

Evaluated by Immunocytochemistry in human RNcell CX neural stem cells (SCC007). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in human RNcell CX neural stem cells (SCC007).

Target description

The uncojugated parent antibody (Catalog No. MAB5326) has an observed MW at 200-220 kDa

Physical form

Protein A purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
RNcell CX neural stem cells (SCC007).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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