MAB5384-I
Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 132.38
clone 132.38, from mouse
Synonym(s):
Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen, NG2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
132.38, monoclonal
species reactivity
mouse, rat
packaging
antibody small pack of 25 μg
technique(s)
immunofluorescence: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
mouse ... Cspg4(121021)
rat ... Cspg4(81651)
General description
Chondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form. (Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).
Specificity
Clone 132.38 is a mouse monoclonal antibody that detects Chondroitin sulfate proteoglycan 4 in rat brain tissue.
Immunogen
HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222.
Application
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38, Cat. No. MAB5384-I, is a highly specific mouse monoclonal antibody that targets Chondrin Sulfate Protoglycan 4 and has been tested for use in Immunofluorescence and Western Blotting.
Research Category
Neuroscience
Neuroscience
Western Blotting Analysis: 1 µg/mL from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in Rat E16 brain tissuel lysate.
Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in rat brain frozen sections, post-fixed in ethanol.
Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in rat brain frozen sections, post-fixed in ethanol.
Quality
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NG2 Chondroitin Sulfate Proteoglycan in NIH/3T3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NG2 Chondroitin Sulfate Proteoglycan in NIH/3T3 cell lysate.
Target description
~260 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: MAB5384 and 05-710
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing PBS, pH 7.2, containing 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mathilde Pruvost et al.
Cell reports, 42(8), 112848-112848 (2023-07-29)
Oligodendrocytes are specialized cells that insulate and support axons with their myelin membrane, allowing proper brain function. Here, we identify lamin A/C (LMNA/C) as essential for transcriptional and functional stability of myelinating oligodendrocytes. We show that LMNA/C levels increase with
Lu-Yao Tong et al.
Frontiers in pharmacology, 13, 914153-914153 (2022-07-23)
The recovery of spinal cord injury (SCI) is closely associated with the obstruction of oligodendrocyte progenitor cell (OPC) differentiation, which ultimately induces the inability to generate newly formed myelin. To address the concern, drug-based methods may be the most practical
Santosh Kumar Samal et al.
Bio-protocol, 14(2), e4921-e4921 (2024-01-25)
The central nervous system (CNS) relies on the complex interaction of neuroglial cells to carry out vital physiological functions. To comprehensively understand the structural and functional interplay between these neuroglial cells, it is essential to establish an appropriate in vitro
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