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MAB5428

Sigma-Aldrich

Anti-Retinal Pigment Epithelium 65 Antibody

clone 401.8B11.3D9, Chemicon®, from mouse

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Synonym(s):
RPE65
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

401.8B11.3D9, monoclonal

species reactivity

bovine, human, Xenopus, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... RPE65(6121)

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This Item
SAB2500895SAB5701703ZRB1640
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

-

antibody form

purified antibody

clone

401.8B11.3D9, monoclonal

clone

polyclonal

clone

monoclonal

clone

2F8, recombinant monoclonal

species reactivity

bovine, human, Xenopus, mouse

species reactivity

human, canine

species reactivity

human, mouse

species reactivity

human, rabbit, mouse

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

packaging

antibody small pack of 25 μL

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

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Specificity

Reacts with Retinal Pigment Epithelium 65 (RPE65). On bovine RPE membranes the antibody recognizes a protein with a molecular weight of ~65 kDa.

Immunogen

Bovine RPE microsomal membranes.

Application

Anti-Retinal Pigment Epithelium 65 Antibody detects level of Retinal Pigment Epithelium 65 & has been published & validated for use in ELISA, IH, IP & WB.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS
Western blot: 1:5,000-1:20,000 on bovine RPE membranes using ECL. Suggested dilution buffer is TBS containing 10% calf serum, 0.25% T-20, 1M D-glucose with 10% glycerol. Suggested blocking buffer is TBS containing 2% BSA and 0.5% Tween 20. Preferred gel percentage is 10%.

Immunohistochemistry on frozen tissue sections: 1:250-1:500

Immunoprecipitation: 20 μg of antibody in a reaction volume of 500 μL.

Immunoaffinity purification

ELISA

Optimal working dilutions must be determined by end user.

Target description

~ 65 kDa

Physical form

Format: Purified
Protein A purified
Purified immunoglobulin. Liquid in PBS. Contains no preservative.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Eye

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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L I Rachek et al.
Endocrinology, 148(1), 293-299 (2006-10-07)
A major characteristic of type 2 diabetes mellitus (T2DM) is insulin resistance in skeletal muscle. A growing body of evidence indicates that oxidative stress that results from increased production of reactive oxygen species and/or reactive nitrogen species leads to insulin
Xiao Yang et al.
eLife, 9 (2020-04-03)
The choroid, which provides vascular supply to the outer retina, demonstrates progressive degeneration in aging and age-related macular degeneration (AMD). However mechanisms that maintain or compromise choroidal homeostasis are obscure. We discovered that the ablation of choroidal macrophages via CSF1R
Glenn Yiu et al.
Molecular therapy. Methods & clinical development, 16, 179-191 (2020-02-15)
Retinal gene therapy using adeno-associated viruses (AAVs) is constrained by the mode of viral vector delivery. Intravitreal AAV injections are impeded by the internal limiting membrane barrier, while subretinal injections require invasive surgery and produce a limited region of therapeutic
Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle.
Mu?iz, A; Greene, WA; Plamper, ML; Choi, JH; Johnson, AJ; Tsin, AT; Wang, HC
Investigative Ophthalmology & Visual Science null
Manabu Hirasawa et al.
The Journal of biological chemistry, 291(14), 7373-7385 (2016-02-04)
Choroidal neovascularization (CNV) is a pathogenic process of age-related macular degeneration, a vision-threatening disease. The retinal pigment epithelium and macrophages both influence CNV development. However, the underlying mechanisms remain obscure. Here, we focus on Angptl2 (angiopoietin-like protein 2), a cytokine

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