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MABC1117

Sigma-Aldrich

Anti-GOLM1 Antibody, clone 6A2

clone 6A2, from mouse

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Synonym(s):
Golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2
eCl@ss:
32160702

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

6A2, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... GOLM1(51280)

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SAB3500305MABT1363MHPA010638
vibrant-m

MABC1117

Anti-GOLM1 Antibody, clone 6A2

vibrant-m

MABT1363M

Anti-GM130 Antibody, clone 4A3

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

Gene Information

human ... GOLM1(51280)

Gene Information

human ... GOLM1(51280)

Gene Information

rat ... Golga2(64528)

Gene Information

-

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

human, rat

species reactivity

human

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:500-1:1000

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General description

Golgi membrane protein 1 (UniProt: Q8NBJ4; also known as Golgi membrane protein GP73, Golgi phosphoprotein 2) is encoded by the GOLM1 (also known as C9orf155, GOLPH2, PSEC0242, UNQ686/PRO1326) gene (Gene ID: 51280) in human. GP73 is a single-pass type II membrane protein that cycles via the cell surface and endosomes upon lumenal pH disruption. It is constitutively expressed in cells of epithelial lineage. It is highly expressed in colon, prostate, trachea, and stomach. In normal liver it is expressed at low level, but the expression is significantly increased in response to viral (HBV, HCV) infections, but not in diseases of non-viral origin. Its levels are also shown to be elevated in hepatocellular carcinoma (HCC) and in invasive breast cancer. GP73 is reported to promote HCC invasion by activation of matrix metalloproteinase-13 (MMP-13). Two isoforms of GP73 have been described that are produced by alternative initiation. (Ref.: Zhanga, A., and Caob, B. (2012). J. Biomed. Res. 26(6); 467-473; Donizy, P., et al. (2016). Int. J. Mol. Sci. 17(10); 1619.

Specificity

Clone 6A2 specifically detects human Golgi membrane protein 1. It targets an epitope with in the lumenal domain.

Immunogen

His-tagged recombinant fragment corresponding to 360 amino acids from the lumenal region of human Golgi membrane protein 1.

Application

Anti-GOLM1, clone 6A2, Cat. No. MABC1117, is a mouse monoclonal antibody that detects Golgi membrane protein 1 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, and Wrstern Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected GOLM1 in human liver cancer tissue sections.

Enzyme Immunoassay Analysis: A representative lot detected GOLM1 in ELISA applications (Zhang, A., et. al. (2012). J Biomed Res. 26(6):467-73).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected GOLM1 in Immunohistochemistry applications (Zhang, A., et. al. (2012). J Biomed Res. 26(6):467-73).

Immunoprecipitation Analysis: A representative lot immunoprecipitated GOLM1 in Immunoprecipitation applications (Zhang, A., et. al. (2012). J Biomed Res. 26(6):467-73).

Western Blotting Analysis: A representative lot detected GOLM1 in Western Blotting applications (Zhang, A., et. al. (2012). J Biomed Res. 26(6):467-73).
Research Category
Apoptosis & Cancer

Quality

Evaluated by Immunohistochemistry (Paraffin) in human prostate cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected GOLM1 in human prostate cancer tissue sections.

Target description

45.33 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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