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MABC1176

Sigma-Aldrich

Anti-Bax Antibody, clone 6A7

from mouse

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Synonym(s):
Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

technique(s)

immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... BAX(581)

General description

Apoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. It displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Upon induction of apoptosis Bax undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. BAX is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 6A7 reacts with multiple isoforms of Bax.

Specificity

Clone 6A7 recognizes BAX in human cells. It targets an epitope with in 13 amino acids from the N-terminal region.

Immunogen

KLH-cojugated linear peptide coprresponding to 13 amino acids from the N-terminal region of human BAX.

Application

Detect using this mouse monoclonal Anti-Bax, clone 6A7 Antibody, Cat. No. MABC1176-25UG, validated for use in Immunoprecipitation, Immunofluorescence and Western Blotting.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: A representative lot detected Bax in Western Blotting applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

Immunofluorescence Analysis: A representative lot detected Bax in Immunofluorescence applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

Immunoprecipitation Analysis:A representative lot detected Bax in Immunoprecipitation applications (Xin, M., et. al. (2014). Nat Commun. 5:4935; Hsu, Y.T., et. al. (1998). J Biol Chem. 273(17):10777-83).

Quality

Evaluated by Immunoprecipitation in THP-1 cell lysates.

Immunoprecipitation Analysis: A 1:250 dilution of this antibody detected Bax in THP-1 cell lysates.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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