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MABC276

Sigma-Aldrich

Anti-BAG3 Antibody, clone AC-2

clone AC-2, from mouse

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Synonym(s):
BAG family molecular chaperone regulator 3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-2, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BAG3(9531)

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MABC282ABC156HPA020586
vibrant-m

MABC276

Anti-BAG3 Antibody, clone AC-2

vibrant-m

MABC282

Anti-BAG3 Antibody, clone AC-1

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

clone

AC-2, monoclonal

clone

AC-1, monoclonal

clone

polyclonal

clone

polyclonal

Gene Information

human ... BAG3(9531)

Gene Information

human ... BAG3(9531)

Gene Information

human ... BAG3(9531)

Gene Information

human ... BAG3(9531)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

UniProt accession no.

O95817

UniProt accession no.

O95817

UniProt accession no.

O95817

UniProt accession no.

O95817

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General description

Bcl2-associated athanogene 3 (BAG3), also known as CAIR-1 or Bis, belongs to a family of six evolutionarily conserved co-chaperone proteins that contain a characteristic C-terminal BAG domain. In most cell types, BAG3 is localized to the cytoplasm, where it regulates the heat shock protein 70 chaperone. BAG3 is activated in response to stressful stimuli, and plays a critical role in modulating multiple cellular processes, including apoptosis, autophagy, embryonic development, and actin rearrangement. Abnormal expression of BAG3 results cardiomyopathy dilated type 1HH.

Immunogen

MAP peptide corresponding to human BAG3.

Application

Anti-BAG3 Antibody, clone AC-2 detects level of BAG3 & has been published & validated for use in Western Blotting.
Immunoprecipitation Analysis: A 1:500 dilution from a representative lot detected BAG3 IP′d from 500 µg of HeLa lysate.
Research Category
Apoptosis & Cancer
Research Sub Category
Chaperones

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected BAG3 in 10 µg of HeLa cell lysate.

Target description

~62-80 kDa observed. The calculated molecular weight of this protein is 62 kDa, but can run as high as 80 kDa due to PTM.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ning Li et al.
BMC cancer, 18(1), 793-793 (2018-08-08)
CRC is one of the most common malignancies worldwide, and its molecular mechanisms remain unclear. Elevated levels of BAG3 have been reported in various tumors. The present study aimed to explore the expression and function of BAG3 in CRC. BAG3

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